植物生理學是研究植物生命活動規(guī)律、揭示植物生命現(xiàn)象本質的一門學科。植
物生理學是農(nóng)學、園藝、植物保護、種子科學與工程、設施農(nóng)業(yè)科學與工程、植物科
學與技術、園林等涉農(nóng)專業(yè)的專業(yè)基礎課程。植物生理學實驗是該課程重要的實踐性
教學環(huán)節(jié)，通過實驗操作能進一步加深學生對理論知識的理解，提高學生動手實踐能
力，將理論和實踐有機結合，為解決農(nóng)業(yè)生產(chǎn)實際問題奠定良好的基礎。
《植物生理學實驗實訓綜合教程》主要包括植物種子的萌發(fā)、水分代謝、礦質元
素缺乏對植物生命活動的影響、植物光合性能的評價、植物碳氮代謝、植物生長物質
的生理效應及其對植物生長發(fā)育的影響、植物組織培養(yǎng)、植物逆境生理等內容，一共
9章。本書基本上涵蓋了植物生理學所有的章節(jié)內容，且充分考慮各章節(jié)的重點、難
點，為我們進一步理解和鞏固相關理論知識提供了幫助。
本書非常注重理論教學和實踐應用的深度結合，由濰坊學院、山東農(nóng)業(yè)工程學
院、濰坊職業(yè)學院和山東省農(nóng)業(yè)技術推廣中心4家單位合作完成，既有本科高校和高
職院校的教師，也有從事農(nóng)業(yè)技術推廣的一線工作人員，他們從不同的專業(yè)角度來合
理安排實驗內容，通過簡單易做的植物實驗來驗證復雜微觀的科學知識，啟發(fā)大家去
探索植物生長的內在規(guī)律，以此提高作物生產(chǎn)潛力。
中文篇
第1章 植物生理學實驗基礎 3
第2章 植物種子的萌發(fā) 14
第3章 植物水分代謝 22
第4章 礦質元素缺乏對植物生命活動的影響 30
第5章 植物光合性能的評價 42
第6章 植物碳氮代謝 59
第7章 植物生長物質的生理效應及其對植物生長發(fā)育的影響 72
第8章 植物組織培養(yǎng) 83
第9章 植物逆境生理 95
附 錄 115
附錄1 相關理論知識鞏固與思考115
附錄2 化學品標志符號202
附錄3 常用酸堿的濃度203
附錄4 常用固態(tài)酸、堿、鹽的物質的量濃度配制參考 203
附錄5 常用緩沖液的配制204
附錄6 常用酸堿指示劑209
附錄7 標準計量單位210
附錄8 常用植物生長物質的一些化學特性212
參考文獻 213
英文篇
Chapter 1 Experimental Basis of Plant Physiology 217
11 Plant physiology laboratory rules217
12 Classification and preparation methods of chemical reagent218
13 Collection, processing and preservation of experimental material220
14 Basic methods of experimental studies on plant physiology228
Chapter 2 Plant Seed Germination 232
21 Determination of seed germination rate, germination potential and
germination index233
22 Rapid determination of seed viability234
23 Determination of seed vigor236
24 The formation and determination of the amylase activity during seed
germination238
25 Changes in fatty acid content of oil seeds during germination239
26 Changes in amino acid content during seed germination241
Chapter 3 Plant Water Metabolism 243
31 Determination of plant leaf water content, relative water content and
water saturation deficit244
32 Determination of free and bound water content in plant leaves
(Malincic method) 245
33 Determination of plant tissue water potential (Small flow method) 247
34 Determination of plant tissue osmotic potential (Plasma wall
separation method) 249
35 Determination of transpiration rate251
Chapter 4 Effects of Mineral Element Deficiency on Plant Life
Activities 254
41 Element deficiency symptoms in plants (solution culture) 255
42 Determination of nitrate reductase activity259
43 Determination of root activity ( - naphthylamine oxidation method) 261
44 Determination of nitrate nitrogen in plants263
45 Determination of plant phosphorus (molybdenum blue method) 265
Chapter 5 Evaluation of Plant Photosynthetic Performance 268
51 Extraction and physicochemical properties of photosynthetic pigments269
52 Separation of photosynthetic pigments and determination of absorption
spectra271
53 Determination of chloroplast pigment content273
54 Determination of plant leaf photosynthetic rate and gas exchange parameters
276
55 Determination of chlorophyll fluorescence parameters280
56 Determination of ribulose diphosphate carboxylase (RuBPCase)
activity286
57 Determination of phosphoenolpyruvate carboxylase (PEPCase)
activity289
Chapter 6 Plant Carbon and Nitrogen Metabolism 291
61 Determination of sucrose, glucose and fructose content292
62 Determination of free amino acid content294
63 Determination of sucrose synthase and sucrose phosphatase activities296
64 Determination of - amylase and - amylase activities298
65 Determination of glutamine synthetase activity302
66 Determination of soluble protein content304
Chapter 7 The Physiological Effects of Plant Growth Substances and
Their Effects on Plant Development 307
71 Effects of auxin on the growth of wheat root and shoot308
72 Effects of gibberellin on the growth of wheat seedlings310
73 Effect of ethephon on the fruit ripening310
74 Abscissive effects of abscisic acid (ABA) on plant petioles311
75 Effects of gibberellin (GA3) and abscisic acid (ABA) on seed
germination313
76 Synthesis of - amylase in seed induced by gibberellin (GA3) 314
77 Effects of abscisic acid (ABA) on stomatal movement317
78 Determination of phytohormones by liquid chromatography318
Chapter 8 Plant Tissue Culture 320
81 Preparation of culture medium321
82 Sterilization, disinfection and inoculation325
83 Subculture and propagation330
84 Acclimation, transplanting and management of tube seedlings334
Chapter 9 Plant Stress Physiology 336
91 Determination of proline content337
92 Determination of total flavonoid content339
93 Determination of ascorbic acid (AsA, AA) 341
94 Determination of glutathione (GsH) 345
95 Determination of cytoplasmic membrane permeability - conductance
method347
96 Identification of degree of membrance peroxidation - determination of
malondialdehyde content (MDA)349
97 Identification of cell death in plant tissues (Trypan blue staining method) 351
98 Determination of hydrogen peroxide (H2O2) content352
99 Determination of superoxide anion production rate354
910 Determination of superoxide dismutase (SOD) activity356
911 Determination of peroxidase (POD) activity358
912 Determination of catalase (CAT) activity360
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